The Evolutionarily Conserved Longevity Determinants HCF-1 and SIR-2.1/SIRT1 Collaborate to Regulate DAF-16/FOXO
2011

How HCF-1 and SIR-2.1 Regulate Lifespan in C. elegans

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Author Information

Author(s): Rizki Gizem, Iwata Terri Naoko, Li Ji, Riedel Christian G., Picard Colette Lafontaine, Jan Max, Murphy Coleen T., Lee Siu Sylvia

Primary Institution: Cornell University

Hypothesis

HCF-1 and SIR-2.1 interact to regulate DAF-16/FOXO activity, influencing lifespan and stress responses.

Conclusion

HCF-1 and SIR-2.1 work together to modulate DAF-16 activity, which is crucial for longevity and stress resistance in both C. elegans and mammals.

Supporting Evidence

  • HCF-1 acts downstream of SIR-2.1 to influence lifespan and oxidative stress response in C. elegans.
  • Genetic analyses showed that hcf-1 mutation leads to a significant lifespan extension.
  • Protein–protein association studies demonstrated that SIR-2.1 and HCF-1 form complexes in both worms and mammalian cells.

Takeaway

Scientists found that two proteins, HCF-1 and SIR-2.1, help control how a gene that affects aging works, which could help us understand how to live longer and healthier.

Methodology

The study used genetic analyses, gene expression profiling, and protein association studies in C. elegans and mammalian cells.

Digital Object Identifier (DOI)

10.1371/journal.pgen.1002235

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