Characterizing Terpene Synthase Genes in Spruce
Author Information
Author(s): Christopher I. Keeling, Sabrina Weisshaar, Steven G. Ralph, Sharon Jancsik, Britta Hamberger, Harpreet K. Dullat, Jörg Bohlmann
Primary Institution: University of British Columbia
Hypothesis
The study aims to identify and functionally characterize the terpene synthase gene family in spruce species.
Conclusion
The study reveals a diverse array of terpene synthases in spruce, contributing to the understanding of chemical diversity in conifer defense mechanisms.
Supporting Evidence
- The study identified at least 69 unique and transcriptionally active terpene synthase genes in spruce.
- Functional characterization revealed 15 monoterpene synthases, 4 sesquiterpene synthases, and 2 diterpene synthases.
- The diversity of terpene synthases parallels the chemical diversity found in spruce oleoresin and volatile emissions.
Takeaway
Spruce trees have special genes that help them make chemicals to protect themselves from bugs and diseases. This study found many of these genes.
Methodology
The study involved transcriptome mining, functional characterization of terpene synthases, and phylogenetic analysis based on expressed sequence tags and full-length cDNAs.
Limitations
The study may not have identified all terpene synthases present in spruce due to the limitations of transcriptome sequencing.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website