Models of Esophageal Cancer Using Patient-Derived Organoids and Fibroblasts
Author Information
Author(s): Sharpe Benjamin P., Nazlamova Liliya A., Tse Carmen, Johnston David A., Thomas Jaya, Blyth Rhianna, Pickering Oliver J., Grace Ben, Harrington Jack, Rajak Rushda, Rose-Zerilli Matthew, Walters Zoe S., Underwood Tim J.
Primary Institution: University of Southampton
Hypothesis
Can patient-derived tumor organoids and cancer-associated fibroblasts be combined to create a model that accurately represents the tumor microenvironment in esophageal adenocarcinoma?
Conclusion
The study successfully developed assembloid models that replicate the tumor microenvironment and interactions between cancer cells and fibroblasts in esophageal adenocarcinoma.
Supporting Evidence
- The assembloid models recapitulate the differentiation status of esophageal adenocarcinoma.
- CAFs support the survival and proliferation of EAC PDOs in culture.
- Whole-mount immunofluorescence allows visualization of tumor-stromal interactions in 3D.
Takeaway
Researchers created a new model of esophageal cancer by mixing cancer cells from patients with supportive cells called fibroblasts, helping to better understand how tumors grow.
Methodology
The study involved co-culturing patient-derived organoids with cancer-associated fibroblasts in a 3D matrix to create assembloid models, followed by characterization using immunofluorescence and histology.
Limitations
The study used heterologous CAFs, which may not fully mimic the interactions present in patient-derived models.
Participant Demographics
Patients with esophageal adenocarcinoma, including both male and female participants aged 60 to 81.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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