How Succinate Affects Endothelial Cell Function
Author Information
Author(s): Reham Atallah, Juergen Gindlhuber, Wolfgang Platzer, Rishi Rajesh, Akos Heinemann
Primary Institution: Medical University of Graz
Hypothesis
Elevated intracellular succinate alters endothelial cell function and barrier integrity.
Conclusion
Succinate significantly regulates endothelial mitochondrial function and barrier integrity, with a response that is counterbalanced by increased VEGF and prostaglandin production.
Supporting Evidence
- Succinate accumulation is linked to various diseases, including cancer and cardiovascular disorders.
- Elevated ROS production was observed in endothelial cells treated with DES.
- Endothelial barrier permeability increased with DES treatment but gradually restored over time.
- COX-2 expression and PGE2 release were increased after DES treatment, contributing to barrier recovery.
- Ascorbic acid pre-treatment reduced the initial barrier drop caused by DES.
Takeaway
When endothelial cells have too much succinate, they can have trouble with their energy and how they stick together, but they can also recover over time.
Methodology
The study used in vitro functional assays with primary human umbilical vein endothelial cells (HUVECs) and diethyl succinate (DES) to assess mitochondrial function and barrier integrity.
Limitations
The study primarily used high concentrations of DES, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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