Is Ciprofloxacin a Substrate of P-glycoprotein?
Author Information
Author(s): Miki Susanto, Okochi Hideaki, Benet Leslie Z
Primary Institution: University of the Pacific
Hypothesis
The study aims to determine if ciprofloxacin is a substrate of P-glycoprotein using MDCKI cells transfected with human MDR1 cDNA.
Conclusion
The study indicates that ciprofloxacin is a substrate of P-glycoprotein in some cell lines but not in others.
Supporting Evidence
- Ciprofloxacin showed net secretion in MDCKI-MDR1 but net absorption in MDCKI cells.
- P-gp inhibitors decreased the B to A transport of ciprofloxacin in MDCKI-MDR1 cells.
- The B to A transport of ciprofloxacin in MDCKI-MDR1 cells was not affected by non-P-gp inhibitors.
Takeaway
The researchers wanted to find out if a medicine called ciprofloxacin is affected by a protein that helps pump drugs out of cells, and they found mixed results depending on the type of cells they used.
Methodology
The study used semi-quantitative RT-PCR and Western blot to analyze mRNA and protein levels, and performed bidirectional transport studies in various cell lines.
Potential Biases
Potential bias may arise from the use of different cell lines and methods to assess P-glycoprotein activity.
Limitations
The study's findings may not be conclusive due to the variability in results across different cell lines.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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