LPS Makes Mice with Human Serum Amyloid P Component More Sensitive to Shiga Toxin 2
Author Information
Author(s): Griener Thomas P., Strecker Jonathan G., Humphries Romney M., Mulvey George L., Fuentealba Carmen, Hancock Robert E. W., Armstrong Glen D.
Primary Institution: University of Calgary
Hypothesis
Does co-administering lipopolysaccharide (LPS) increase the susceptibility of HuSAP-transgenic mice to Shiga toxin 2 (Stx2)?
Conclusion
Co-administering LPS with Stx2 increases the susceptibility of HuSAP-transgenic mice to the lethal effects of Stx2.
Supporting Evidence
- HuSAP-transgenic mice are resistant to Stx2, but LPS treatment reverses this resistance.
- LPS treatment increases expression of pro-inflammatory cytokines in the kidneys.
- Dexamethasone protects LPS-sensitized mice from lethal doses of Stx2.
Takeaway
When mice that usually resist a harmful toxin are given a certain substance, they become more vulnerable to that toxin.
Methodology
Transgenic mice were injected with Stx2 and/or LPS, and their survival and immune responses were monitored.
Limitations
The study was conducted in mice, which may not fully replicate human responses.
Participant Demographics
C57BL/6-Tg(APSC)1Imeg transgenic mice, both male and female, aged 8-10 weeks.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website