ZNF198 Stabilizes the LSD1–CoREST–HDAC1 Complex on Chromatin
Author Information
Author(s): Gocke Christian B., Yu Hongtao
Primary Institution: Howard Hughes Medical Institute, Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
Hypothesis
ZNF198-like proteins are required for the repression of E-cadherin but not REST-responsive genes.
Conclusion
ZNF198-like proteins help maintain the integrity of the LSD1–CoREST–HDAC1 complex on specific promoters and may prevent the dissociation of HDAC1.
Supporting Evidence
- ZNF198-like proteins are required for the repression of E-cadherin, a gene known to be repressed by LSD1.
- Depletion of ZNF198-like proteins weakens the association of the LSD1–CoREST–HDAC1 complex with chromatin.
- ZNF198 binds preferentially to the intact LSD1–CoREST–HDAC1 ternary complex.
Takeaway
ZNF198 is a protein that helps keep other proteins together to turn off certain genes, like E-cadherin, but doesn't help with all genes.
Methodology
The study used RNA interference to deplete ZNF198-like proteins in HeLa cells and analyzed the effects on gene expression and protein interactions.
Limitations
The study did not explore the role of ZNF198-like proteins in the repression of all LSD1 target genes.
Digital Object Identifier (DOI)
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