Human Neural Progenitors and Lysophospholipid Receptors
Author Information
Author(s): Hurst Jillian H, Mumaw Jennifer, Machacek David W, Sturkie Carla, Callihan Phillip, Stice Steve L, Hooks Shelley B
Primary Institution: University of Georgia
Hypothesis
Do hES-NEP cells express functional lysophospholipid receptors that mediate cellular responses critical for neural development?
Conclusion
Lysophospholipids regulate neural progenitor growth and morphology through distinct mechanisms.
Supporting Evidence
- Lysophosphatidic Acid (LPA) and Sphingosine-1-phosphate (S1P) receptors are functionally expressed in hES-NEP cells.
- Transcript levels for S1P1 receptor increased significantly in the transition from embryonic stem cell to hES-NEP.
- LPA and S1P stimulate cell proliferation via Gi/o coupled receptors in an Epidermal Growth Factor Receptor (EGFR)- and ERK-dependent pathway.
- LPA and S1P induce reversible morphological changes in hES-NEP cells.
Takeaway
This study shows that certain fats called lysophospholipids help brain cells grow and change shape in different ways.
Methodology
The study involved establishing a stable human embryonic stem cell-derived neuroepithelial cell line and assessing the expression and function of lysophospholipid receptors through various assays.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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