KAP1 Binding and Autoregulation of KRAB-ZNFs
Author Information
Author(s): O'Geen Henriette, Squazzo Sharon L, Iyengar Sushma, Blahnik Kim, Rinn John L, Chang Howard Y, Green Roland, Farnham Peggy J
Primary Institution: University of California Davis
Hypothesis
Does KAP1 mediate the trimethylation of histone H3 on lysine 9 and regulate the expression of KRAB-ZNF genes?
Conclusion
KAP1 is involved in the autoregulation of KRAB-ZNF gene expression through its binding to their transcribed regions.
Supporting Evidence
- KAP1 binding sites were identified in the transcribed regions of ZNF genes.
- H3me3K9 and H3me3K27 target different classes of transcription factors.
- Many KAP1 target genes are also bound by H3me3K9.
- ZNF genes are the most highly enriched class of KAP1 target genes.
- KAP1 represses transcription of many KRAB-ZNF genes.
Takeaway
This study found that a protein called KAP1 helps control the activity of certain genes by sticking to them, especially those that are important for making other proteins.
Methodology
The study used chromatin immunoprecipitation (ChIP)-chip assays to analyze KAP1 binding across ~26,000 human promoters.
Statistical Information
P-Value
5E−27
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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