HIV-1 Vpr-mediated G2 arrest involves the DDB1-CUL4AVPRBP E3 ubiquitin ligase
2007
HIV-1 Vpr and G2 Cell Cycle Arrest
publication
Evidence: moderate
Author Information
Author(s): Belzile Jean-Philippe, Duisit Ghislaine, Rougeau Nicole, Mercier Johanne, Finzi Andrés, Cohen Éric A
Primary Institution: Institut de Recherches Cliniques de Montréal, Montreal, Quebec, Canada
Hypothesis
How does HIV-1 Vpr induce G2 cell cycle arrest in human cells?
Conclusion
The study identifies a complex involving Vpr, DDB1, and VPRBP that is necessary for Vpr-mediated G2 arrest.
Supporting Evidence
- Vpr interacts with DDB1 and VPRBP, which are part of an E3 ubiquitin ligase complex.
- Depletion of VPRBP impairs Vpr-mediated G2 arrest without affecting normal cell cycle progression.
- Vpr's ability to induce G2 arrest is linked to its interaction with the DDB1-CUL4AVPRBP complex.
Takeaway
HIV-1 Vpr is a protein that can stop cells from dividing, and it does this by working with other proteins to affect the cell cycle.
Methodology
The study used tandem affinity purification and mass spectrometry to identify proteins interacting with Vpr.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website