Restoration of Bone Formation in Aged Mice Using TNF Blockers
Author Information
Author(s): Wahl Elizabeth C, Aronson James, Liu Lichu, Fowlkes John L, Thrailkill Kathryn M, Bunn Robert C, Skinner Robert A, Miller Mike J, Cockrell Gael E, Clark Lindsey M, Ou Yang, Isales Carlos M, Badger Thomas M, Ronis Martin J, Sims John, Lumpkin Charles K Jr
Primary Institution: University of Arkansas for Medical Sciences
Hypothesis
Elevated levels of TNF associated with aged animals mediate the age-related inhibition of direct bone formation.
Conclusion
The study found that TNF blockers significantly improved bone formation in aged mice.
Supporting Evidence
- TNF blockers increased the mineralized area of distraction gaps in aged mice.
- 86% of the sTNFR1-treated aged mice bridged the gap during distraction.
- Histologic analysis confirmed increased bone formation in treated aged mice.
- Serum analyses showed elevated levels of TNF and IL-6 in aged versus young mice.
- p21 knockout mice were resistant to the osteoinhibitory effects of TNF.
Takeaway
Older mice have trouble healing bones, but using certain medicines can help them heal better.
Methodology
The study used a distraction osteogenesis model in mice to test the effects of TNF blockers on bone formation.
Limitations
The study was conducted on mice, which may not fully represent human responses.
Participant Demographics
Adult male C57BL/6 mice, aged 3 months and 22 months.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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