Identifying Secreted Proteins from the Anthracnose Pathogen Colletotrichum truncatum
Author Information
Author(s): Bhadauria Vijai, Banniza Sabine, Vandenberg Albert, Selvaraj Gopalan, Wei Yangdou
Primary Institution: University of Saskatchewan
Hypothesis
The study aims to identify proteins that are secreted during the biotrophy-necrotrophy switch of Colletotrichum truncatum.
Conclusion
The study catalogs proteins that may play a role in establishing a compatible interaction with the host plant during the infection process.
Supporting Evidence
- Nearly 39% of the ESTs were predicted to encode proteins of fungal origin.
- 162 ESTs were predicted to contain N-terminal signal peptides.
- Eleven candidate effector genes were identified based on features common to characterized fungal effectors.
Takeaway
Scientists looked at proteins made by a fungus that causes disease in plants to understand how it infects them.
Methodology
A directional cDNA library was constructed from mRNA isolated from infected lentil tissues, generating 5000 expressed sequence tags (ESTs) for analysis.
Participant Demographics
Infected lentil cultivar 'Eston' was used for the study.
Digital Object Identifier (DOI)
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