Structure of fructose bisphosphate aldolase from Encephalitozoon cuniculi
2011
Structure of Fructose Bisphosphate Aldolase from E. cuniculi
publication
Evidence: high
Author Information
Author(s): Anna Gardberg, Banumathi Sankaran, Doug Davies, Janhavi Bhandari, Bart Staker, Lance Stewart
Primary Institution: Emerald BioStructures
Conclusion
The study determined the crystal structures of fructose bisphosphate aldolase from E. cuniculi, revealing its dimeric structure and substrate-binding conformation.
Supporting Evidence
- The enzyme crystallizes readily in the presence of phosphate.
- The structure was refined to a final free R factor of 20.8%.
- The dimeric structure was unexpected compared to other aldolases.
Takeaway
Scientists looked at a special enzyme from a tiny parasite and found out how it works with sugar. They discovered it has a different shape than expected.
Methodology
The enzyme was purified and crystallized, and its structure was analyzed using X-ray diffraction.
Digital Object Identifier (DOI)
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