L1TD1 as a Marker for Undifferentiated Human Embryonic Stem Cells
Author Information
Author(s): Wong Raymond Ching-Bong, Ibrahim Abel, Fong Helen, Thompson Noelle, Lock Leslie F., Donovan Peter J.
Primary Institution: University of California Irvine
Hypothesis
Is L1TD1 a useful marker for identifying undifferentiated human embryonic stem cells?
Conclusion
L1TD1 is a downstream target of Nanog and serves as a useful marker for identifying undifferentiated human embryonic stem cells.
Supporting Evidence
- L1TD1 is highly expressed in undifferentiated human embryonic stem cells.
- L1TD1 expression is rapidly downregulated upon differentiation of stem cells.
- Nanog is associated with the promoter region of L1TD1, indicating regulatory control.
Takeaway
L1TD1 is a special marker that helps scientists find undifferentiated stem cells, which can turn into any type of cell in the body.
Methodology
The study used quantitative PCR, western blot analysis, and chromatin immunoprecipitation to analyze L1TD1 expression in human embryonic stem cells.
Limitations
The study does not address the functional role of L1TD1 in detail.
Digital Object Identifier (DOI)
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