Androgen receptor protein is down-regulated by basic fibroblast growth factor in prostate cancer cells
2000
How basic fibroblast growth factor affects androgen receptor in prostate cancer cells
publication
Evidence: moderate
Author Information
Author(s): Cronauer M V, Nessler-Menardi C, Klocker H, Maly K, Hobisch A, Bartsch G, Culig Z
Primary Institution: University of Innsbruck
Hypothesis
Does basic fibroblast growth factor (bFGF) down-regulate androgen receptor (AR) expression in prostate cancer cells?
Conclusion
Basic fibroblast growth factor significantly reduces androgen receptor protein expression in prostate cancer cells.
Supporting Evidence
- bFGF diminished prostate-specific antigen protein in the supernatants of androgen-stimulated human prostate cancer cells LNCaP by 80%.
- bFGF down-regulated AR protein in a dose-dependent manner showing a maximal effect at 50 ng ml−1.
- Down-regulation of AR protein expression occurred already after 8 h of bFGF treatment.
- A maximal inhibition of AR protein was observed 24 h after addition of bFGF to culture media.
- Calcium release from intracellular stores was measured to investigate the mechanism of bFGF's effect.
Takeaway
This study found that a substance called basic fibroblast growth factor can lower the amount of a protein that helps prostate cancer cells grow.
Methodology
LNCaP prostate cancer cells were treated with bFGF, and AR protein expression was measured using immunoblotting and ligand binding assays.
Digital Object Identifier (DOI)
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