HPLC Method for Measuring Losartan and Metolazone
Author Information
Author(s): Dubey Ramkumar, Bhusari Vidhya K., Dhaneshwar Sunil R.
Primary Institution: Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India
Hypothesis
This study aims to develop and validate a method for the simultaneous quantitation of Losartan potassium and Metolazone using HPLC.
Conclusion
The developed HPLC method is effective for the simultaneous quantitation of Losartan potassium and Metolazone in pharmaceutical formulations.
Supporting Evidence
- The method showed good linearity for both drugs over specified concentration ranges.
- Precision studies indicated RSD values were less than 2%, meeting ICH guidelines.
- The method was validated for accuracy with recoveries between 99% and 101%.
Takeaway
Researchers created a new way to measure two heart medications together using a special machine called HPLC, which helps doctors know how much medicine is in a pill.
Methodology
The method involves HPLC separation of Losartan potassium and Metolazone using a specific column and mobile phase, followed by validation for precision, accuracy, and robustness.
Digital Object Identifier (DOI)
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