Glutathione and Ophthalmate Levels in Woodchucks with Hepatocellular Carcinoma
Author Information
Author(s): Andres Ibarra Rafael, Abbas R., Kombu R. S., Zhang Guo-Fang, Jacobs G., Lee Z., Brunengraber H., Sanabria J. R.
Primary Institution: Case Western Reserve University School of Medicine and University Hospitals, Case Medical Center
Hypothesis
Can disturbances in the glutathione redox buffer system be detected in animals with chronic viral hepatitis-induced HCC?
Conclusion
The study found significant disturbances in the glutathione redox buffer system and higher concentrations of ophthalmate in woodchucks with hepatitis virus-induced HCC.
Supporting Evidence
- The concentration of reduced glutathione was lower in plasma from WHV+/HCC+ animals compared to WHV+/HCC− and WHV−/HCC−.
- The concentration of oxidized glutathione was higher in plasma from WHV+/HCC+ animals compared to WHV+/HCC− and WHV−/HCC−.
- Ophthalmate concentration was significantly higher in WHV+/HCC+ compared to WHV+/HCC− and WHV−/HCC−.
Takeaway
This study looked at how certain chemicals in the blood change when woodchucks have liver cancer caused by a virus, which might help doctors find liver cancer earlier in people.
Methodology
Glutathione species and ophthalmate concentrations were measured in plasma and red blood cells from infected woodchucks using LC-MS.
Limitations
The sample size was limited and one animal was excluded due to illness; the correlation of tumor volume with glutathione disturbances was not explored.
Participant Demographics
Woodchucks aged 21–36 months, weighing 2.5–2.75 kg.
Statistical Information
P-Value
0.0002
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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