Comparing Methods for Dissociating Stem Cells
Author Information
Author(s): Heng Boon C, Cowan Catherine M, Basu Shubhayu
Primary Institution: Abbott Vascular
Hypothesis
Is enzyme-free dissociation as effective as trypsin for mesenchymal stem cells?
Conclusion
Dissociating mesenchymal stem cells with enzyme-free buffers results in significantly lower cell viability and reattachment compared to trypsin.
Supporting Evidence
- Cell viability was significantly higher with trypsin compared to enzyme-free buffers.
- The proportion of viable reattached cells was also significantly higher with trypsin.
- Exposure to enzyme-free buffers did not significantly affect cell viability after dissociation.
Takeaway
This study found that using trypsin to separate stem cells is much better than using enzyme-free solutions, which can hurt the cells.
Methodology
The study compared cell viability and reattachment rates of mesenchymal stem cells dissociated with trypsin versus enzyme-free buffers using trypan blue exclusion and MTT assays.
Limitations
The study did not explore the underlying mechanisms for the differences in cell viability between the two dissociation methods.
Participant Demographics
Bone marrow-derived human mesenchymal stem cells.
Statistical Information
P-Value
p = 0.002
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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