Evaluation of Red Cell Hemolysis in Packed Red Cells During Processing and Storage
Author Information
Author(s): R. N. Makroo, Vimarsh Raina, Aakanksha Gupta, Richa Majid, Abdul Thakur, Uday Kumar, Rosamma N. L.
Primary Institution: Indraprastha Apollo Hospitals
Hypothesis
The study aims to evaluate the extent of RBC hemolysis with storage and the effect of leucoreduction and composition of the additive solution on the same.
Conclusion
Hemolysis of red cells increases with storage, but it does not exceed permissible limits at any time, indicating effective processing and storage conditions.
Supporting Evidence
- Percentage hemolysis progressively increases with the storage period in all stored red cell units.
- Free hemoglobin in all red cell units was within permissible levels even on the 42nd day of storage.
- The mean percentage hemolysis was slightly higher in SAGM bags compared to ADSOL bags, but the difference was not statistically significant.
Takeaway
When blood cells are stored, they can break down a little, but this study shows that they stay safe to use even after a long time.
Methodology
Eighty units of packed red cells were evaluated for plasma hemoglobin at various time points during storage using HemoCue and Beckman Coulter analyzers.
Limitations
The study was conducted at a single center, which may limit the generalizability of the findings.
Digital Object Identifier (DOI)
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