Flow Cytometry of Human Primary Epidermal and Follicular Keratinocytes
Author Information
Author(s): Gragnani Alfredo MD, PhD, Polito Michelle Zampieri, Sobral Christiane S MD, PhD, Brunialti Milena Karina Coló PhD, Salomão Reinaldo MD, Ferreira Lydia Masako MD
Primary Institution: Federal University of São Paulo (UNIFESP), São Paulo, Brazil
Hypothesis
The study aimed to characterize cultured human primary keratinocytes isolated from the epidermis and hair follicles by different methods.
Conclusion
The isolation method of treatment with dispase for 18 hours and with trypsin for 10 minutes produced the largest number of viable cells and the smallest number of cells in apoptosis/necrosis.
Supporting Evidence
- The method using dispase for 18 hours and trypsin for 10 minutes yielded the highest number of viable cells.
- Statistical analysis showed significant differences in cell viability between the different treatment groups.
- Flow cytometry allowed for detailed analysis of cell characteristics and viability.
Takeaway
The best way to get healthy skin cells from hair and skin is to use a special method that helps more cells stay alive.
Methodology
Human keratinocytes were isolated from skin and hair follicles using three different enzyme treatments and analyzed using flow cytometry.
Limitations
The study focused on a specific age range (0-15 years) and may not be generalizable to other age groups.
Participant Demographics
Patients aged between 0 and 15 years, with skin fragments obtained from surgical procedures.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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