Low-Level Laser Therapy Activates NF-kB in Mouse Cells
Author Information
Author(s): Chen Aaron C-H., Arany Praveen R., Huang Ying-Ying, Tomkinson Elizabeth M., Sharma Sulbha K., Kharkwal Gitika B., Saleem Taimur, Mooney David, Yull Fiona E., Blackwell Timothy S., Hamblin Michael R.
Primary Institution: Wellman Center for Photomedicine, Massachusetts General Hospital
Hypothesis
Does low-level laser therapy activate NF-kB in mouse embryonic fibroblasts?
Conclusion
Low-level laser therapy enhances mitochondrial respiration and activates NF-kB signaling through the generation of reactive oxygen species.
Supporting Evidence
- LLLT activated NF-kB earlier than conventional treatments.
- Reactive oxygen species were produced in response to laser exposure.
- Antioxidants inhibited NF-kB activation induced by LLLT.
- LLLT increased ATP levels in cells.
Takeaway
This study shows that shining a special laser light on mouse cells helps them produce energy and activates a protein that helps cells survive stress.
Methodology
The study involved isolating mouse embryonic fibroblasts and exposing them to 810 nm laser radiation to measure NF-kB activation and reactive oxygen species production.
Limitations
The study only demonstrated laser-induced NF-kB activation in fibroblasts, which may not represent other cell types.
Participant Demographics
Mouse embryonic fibroblasts from transgenic NF-kB luciferase reporter mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website