High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
Author Information
Author(s): Sandra L. Spurgeon, Robert C. Jones, Ramesh Ramakrishnan
Primary Institution: Fluidigm Corporation
Hypothesis
Can microfluidic dynamic arrays improve the throughput and reliability of gene expression measurements compared to conventional methods?
Conclusion
The microfluidic dynamic array chips provide a rapid and reliable method for high throughput gene expression analysis, showing excellent concordance with conventional real-time PCR.
Supporting Evidence
- The microfluidic dynamic array allows for 2,304 simultaneous real-time PCR measurements.
- The data obtained showed better reproducibility than commercial DNA microarrays.
- The results were validated against conventional real-time PCR and microarray data.
Takeaway
This study shows that a special chip can measure many genes at once, making it easier and faster to see how genes work in different tissues.
Methodology
The study used microfluidic dynamic arrays to perform real-time PCR on cDNA from 18 different human tissues, measuring the expression of 45 genes simultaneously.
Limitations
The study may have limitations related to the specific tissues and genes analyzed, and the generalizability of the findings to other contexts.
Participant Demographics
The study involved cDNA samples from 18 different human tissues.
Digital Object Identifier (DOI)
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