IDO Expression in CHO Cells Affects T Cell Apoptosis and Treg Proportion
Author Information
Author(s): Sun Jingyan, Yu Jinpu, Li Hui, Yang Lili, Wei Feng, Yu Wenwen, Liu Juntian, Ren Xiubao
Primary Institution: Tianjin Medical University Cancer Institute and Hospital
Hypothesis
IDO may contribute to the differentiation of new T regulatory cells from naive CD4+ T cells in breast cancer.
Conclusion
IDO helps create a tolerogenic environment in breast tumors by inducing T cell apoptosis and enhancing Treg-mediated immunosuppression.
Supporting Evidence
- 79.07% of CD3+ T cells underwent apoptosis after co-culture with IDO+ CHO cells.
- The proportion of Tregs increased from 3.43% to 8.98% after co-culture with IDO+ CHO cells.
- 1-MT, an IDO inhibitor, significantly reduced T cell apoptosis.
- Foxp3 expression was confirmed to be higher in T cells co-cultured with IDO+ CHO cells.
Takeaway
The study found that a protein called IDO can make certain immune cells die and increase the number of other cells that help tumors grow.
Methodology
CHO cells were transfected with IDO and co-cultured with CD3+ T cells from breast cancer patients to assess apoptosis and Treg proportions.
Potential Biases
Potential bias in patient selection and the use of a single cell line.
Limitations
The study was conducted in vitro, which may not fully represent in vivo conditions.
Participant Demographics
Breast cancer patients, specific demographics not detailed.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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