HCV Genotypes in Chronic Infection Patients from Rondônia, Brazil
Author Information
Author(s): Vieira Deusilene S, Alvarado-Mora Mónica V, Botelho Lívia, Carrilho Flair J, Pinho João RR, Salcedo Juan M
Primary Institution: Laboratory of Tropical Gastroenterology and Hepatology, São Paulo Institute of Tropical Medicine and Department of Gastroenterology, School of Medicine, University of São Paulo
Hypothesis
What are the HCV genotypes present in chronic infection patients from Rondônia, Brazil?
Conclusion
Subtype 1b was found to be the most prevalent HCV genotype in patients from Rondônia State.
Supporting Evidence
- 22 out of 58 anti-HCV positive samples were successfully sequenced.
- Genotype 1b was the most prevalent, found in 50% of the samples.
- Other genotypes identified included 1a (27.2%), 2b (13.6%), and 3a (9.0%).
- This is the first report of HCV genotypes from Rondônia State.
Takeaway
This study looked at people in Rondônia, Brazil, to see what types of hepatitis C virus they had, and found that the most common type was 1b.
Methodology
The study used nested PCR to amplify a fragment of the NS5B region for genotyping analysis and conducted phylogenetic analysis using Bayesian methods.
Limitations
The study had a small sample size of 22 successfully sequenced samples, limiting the ability to make broader inferences about HCV genetic variability.
Participant Demographics
29 males and 29 females, ages ranging from 27 to 85 years.
Digital Object Identifier (DOI)
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