A Comparative Study of Mouse Hepatic and Intestinal Gene Expression Profiles under PPARα Knockout by Gene Set Enrichment Analysis
2011
Comparative Study of Gene Expression in Mouse Liver and Intestine
Sample size: 28
publication
10 minutes
Evidence: moderate
Author Information
Author(s): He Kan, Wang Qishan, Yang Yumei, Wang Minghui, Pan Yuchun
Primary Institution: Shanghai Jiao Tong University
Hypothesis
What are the tissue-specific pathways or genes involved in PPARα activation in mouse liver and intestine?
Conclusion
The study found that PPARα activation in the mouse small intestine involves more complex regulatory mechanisms compared to the liver.
Supporting Evidence
- The study identified 12 PPARα-dependent pathways common in both liver and intestine.
- More pathways were affected in the intestine than in the liver, indicating a more complex regulatory mechanism.
- Several cancer-related pathways were identified, suggesting a role for PPARα in cancer prevention.
Takeaway
This study looked at how a specific protein affects gene activity in the liver and intestines of mice, finding that it works differently in each place.
Methodology
Gene set enrichment analysis was applied to two microarray datasets related to PPARα in mouse liver and intestine.
Participant Demographics
Mice, specifically wild type and PPARα-null strains.
Statistical Information
P-Value
≤0.01
Statistical Significance
p≤0.01
Digital Object Identifier (DOI)
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