Function and Assembly of a Chromatin-Associated RNase P that Is Required for Efficient Transcription by RNA Polymerase I
2008
Role of RNase P in Transcription by RNA Polymerase I
publication
Evidence: high
Author Information
Author(s): Reiner Robert, Krasnov-Yoeli Natalie, Dehtiar Yana, Jarrous Nayef
Primary Institution: Department of Molecular Biology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
Hypothesis
Human RNase P is required for efficient transcription of rDNA by RNA Polymerase I.
Conclusion
The study reveals that RNase P activates transcription of rDNA by Pol I through a novel assembly process.
Supporting Evidence
- Knockdown of RNase P subunits inhibits transcription of rDNA by Pol I.
- RNase P associates with components of Pol I and its transcription factors.
- Chromatin occupancy by RNase P is linked to the cell cycle.
Takeaway
RNase P helps make the instructions for making proteins in cells by working with a special machine called RNA Polymerase I.
Methodology
The study used biochemical and reverse genetic methods to analyze the role of RNase P in transcription.
Digital Object Identifier (DOI)
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