Genetic Variability in Mycobacterium tuberculosis mce Operons
Author Information
Author(s): Pasricha Rashmi, Chandolia Amita, Ponnan Prija, Saini Neeraj Kumar, Sharma Sangeeta, Chopra Madhu, Basil Mandira Varma, Brahmachari Vani, Bose Mridula
Primary Institution: Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Hypothesis
What is the extent of polymorphism in the mce1 and mce4 operons of Mycobacterium tuberculosis?
Conclusion
The study found that SNPs in the mce1 and mce4 operons of Mycobacterium tuberculosis are significantly high, with the mce4 operon being more polymorphic than mce1.
Supporting Evidence
- 20 single nucleotide polymorphisms (SNPs) were discovered in the mce1 and mce4 operons.
- 12 of the 20 SNPs were nonsynonymous.
- 5 nonsynonymous SNPs were predicted to be deleterious.
- mce4 operon is significantly more polymorphic than mce1 operon.
Takeaway
Scientists looked at the genes of a bacteria that causes tuberculosis and found many tiny changes that could affect how the bacteria behaves.
Methodology
The study analyzed SNPs in mce1 and mce4 operons from 112 clinical isolates and four standard reference strains using direct sequencing and Sequenom MassARRAY platform.
Participant Demographics
112 clinical isolates from patients visiting the outpatient department of Vallabhbhai Patel Chest Institute, Delhi, India.
Statistical Information
P-Value
p < 0.001
Statistical Significance
p < 0.001
Digital Object Identifier (DOI)
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