The GET Complex and Tail-Anchored Proteins
Author Information
Author(s): Schuldiner Maya, Metz Jutta, Schmid Volker, Denic Vladimir, Rakwalska Magdalena, Schmitt Hans Dieter, Schwappach Blanche, Weissman Jonathan S.
Primary Institution: Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco
Hypothesis
How does the GET complex mediate the insertion of tail-anchored proteins into the ER membrane?
Conclusion
The GET complex is essential for the proper insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
Supporting Evidence
- Get3 specifically recognizes tail-anchored proteins destined for the secretory pathway.
- Absence of Get1/Get2 leads to aggregation of Get3-TA complexes.
- In vitro reconstitution shows that the GET proteins mediate insertion of TA proteins into ER membranes.
Takeaway
The GET complex helps proteins that are anchored to membranes find the right place in the cell, like a mailman delivering packages to the correct houses.
Methodology
The study used both in vivo and in vitro experiments to analyze the role of the GET complex in protein insertion.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website