Adaptor SKAP-55 Binds p21ras Activating Exchange Factor RasGRP1 and Negatively Regulates the p21ras-ERK Pathway in T-Cells
2008
How SKAP-55 Affects T-Cell Signaling
publication
Evidence: high
Author Information
Author(s): Helga Schneider, Hongyan Wang, Monika Raab, Elke Valk, Xin Smith, Matt Lovatt, Zhonglin Wu, Braudel Maqueira-Iglesias, Klaus Strebhardt, Christopher E. Rudd
Primary Institution: University of Cambridge
Hypothesis
SKAP-55 may regulate T-cell signaling by affecting the p21ras-ERK pathway and T-cell adhesion.
Conclusion
SKAP-55 negatively regulates the p21ras-ERK pathway while positively regulating T-cell adhesion.
Supporting Evidence
- SKAP-55 deficient T-cells showed impaired adhesion but increased ERK activation.
- RNAi knockdown of SKAP-55 in T-cell lines resulted in enhanced p21ras activation.
- Over-expression of SKAP-55 inhibited ERK activation and its downstream target ELK.
Takeaway
SKAP-55 helps T-cells stick to other cells and controls how they respond to signals, like a traffic light for their activity.
Methodology
The study involved experiments with SKAP-55 deficient T-cells, RNAi knockdown in T-cell lines, and over-expression studies to assess ERK and p21ras activation.
Digital Object Identifier (DOI)
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