Improving siRNA Conjugation with Albumin
Author Information
Author(s): Lau Shannen, Graham Bim, Boyd Ben J., Pouton Colin W., White Paul J.
Primary Institution: Monash Institute of Pharmaceutical Sciences, Monash University
Hypothesis
Commercially supplied amine-modified siRNAs may require ultrafiltration prior to conjugation with amine-reactive compounds.
Conclusion
Ultrafiltration significantly increases the yield of siRNA-albumin conjugates by removing interfering ammonium ions.
Supporting Evidence
- Ultrafiltration increased the yield of siRNA-albumin conjugate by approximately 15-fold.
- Ammonium ions from the purification process interfered with the conjugation reaction.
- Reducing the SMCC to siRNA ratio did not significantly affect conjugate yield after ultrafiltration.
Takeaway
If you want to attach a special tag to a tiny piece of genetic material, you might need to clean it first to make sure it sticks properly.
Methodology
The study involved conjugating siRNA to bovine serum albumin using a linker and tested the effects of ultrafiltration on conjugation efficiency.
Limitations
The study primarily focused on one type of siRNA and its interaction with a specific linker and protein.
Digital Object Identifier (DOI)
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