Effects of Dielectrophoresis on Listeria monocytogenes
Author Information
Author(s): Yang Liju, Banada Padmapriya P, Bhunia Arun K, Bashir Rashid
Primary Institution: North Carolina Central University
Hypothesis
Does dielectrophoresis (DEP) manipulation affect the growth, viability, and immuno-reactivity of Listeria monocytogenes cells?
Conclusion
DEP treatment can enhance the immuno-reactivity of Listeria monocytogenes cells to certain antibodies but prolonged treatment negatively affects cell viability and growth.
Supporting Evidence
- A 1-h DEP treatment increased cell immuno-reactivity to KPL antibodies by ~31.8% and to C11E9 antibodies by ~82.9%.
- Prolonged DEP treatment (4 h or greater) caused significant delays in cell growth.
- 56.8–89.7% reduction in viable cell numbers was observed after 4-h DEP treatment.
Takeaway
This study shows that using electricity to move Listeria bacteria can help them stick to antibodies better, but if you use it for too long, it can hurt the bacteria.
Methodology
The study used ELISA tests to measure immuno-reactivity, pH measurements for growth profiles, and plating methods for cell viability.
Limitations
The study primarily focused on the effects of DEP on Listeria monocytogenes and may not be generalizable to other bacteria.
Statistical Information
P-Value
p<0.0002 for KPL antibody; p<0.009 for C11E9 antibody
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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