Comparing Splicing Isoforms in Human and Mouse Genes
Author Information
Author(s): Federico Zambelli, Giulio Pavesi, Carmela Gissi, David S Horner, Graziano Pesole
Primary Institution: Università degli Studi di Milano
Hypothesis
To assess the extent to which traditional gene annotations capture genuine splicing orthology between human and mouse genes.
Conclusion
The study found that 32% of human and mouse orthologous genes share an identical exon/intron structure, but about 13% of human RefSeq annotated genes lack a corresponding mouse transcript with sufficient similarity.
Supporting Evidence
- 32% of human and mouse orthologous genes have identical exon/intron structures.
- 87% of genes meet less stringent criteria for structural similarity.
- 13% of human RefSeq annotated genes lack a corresponding mouse transcript.
- 25% of human transcripts do not have an orthologous splicing counterpart in mouse.
- 10% of human genes remain without any orthologous splicing variant even with ASPicDB data.
Takeaway
Scientists looked at genes in humans and mice to see how many of their parts are the same, and they found that many genes are similar, but some are different.
Methodology
The study compared the exon-intron structures of human and mouse genes using the Exalign algorithm and analyzed RefSeq and ASPicDB transcript collections.
Limitations
The study's findings are limited by the incompleteness of current gene and transcript data, which may not capture all splicing variants.
Digital Object Identifier (DOI)
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