Recurrent Recruitment Manoeuvres Improve Lung Mechanics in Mice During Mechanical Ventilation
Author Information
Author(s): Reiss Lucy Kathleen, Kowallik Anke, Uhlig Stefan, Hartl Dominik
Primary Institution: Institute of Pharmacology and Toxicology, Medical Faculty of RWTH Aachen University, Aachen, Germany
Hypothesis
The study aims to establish experimental conditions for stable mechanical ventilation in mice and to examine the effects of frequent recruitment manoeuvres on lung mechanics.
Conclusion
Recurrent recruitment manoeuvres maintain lung mechanics in their physiological range during low tidal volume ventilation of healthy mice by preventing atelectasis and reducing pulmonary inflammation.
Supporting Evidence
- Mechanical ventilation without recruitment manoeuvres worsened lung mechanics due to alveolar collapse.
- Recurrent recruitment manoeuvres improved lung mechanics and reduced pulmonary inflammation.
- Higher positive end-expiratory pressure was beneficial for lung mechanics and oxygenation.
Takeaway
This study shows that doing special breathing exercises (recruitment manoeuvres) helps keep the lungs of mice healthy during mechanical ventilation.
Methodology
Mice were ventilated at low and high tidal volumes with different levels of positive end-expiratory pressure and recruitment manoeuvres were performed every 5 or 60 minutes.
Potential Biases
Potential bias in the selection of ventilation strategies and monitoring parameters.
Limitations
The study was conducted on healthy mice, and results may not be directly applicable to humans or other conditions.
Participant Demographics
Female C57BL/6 N mice, aged 8 to 12 weeks, weighing 20 to 25 grams.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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