The effects of secretome of umbilical cord mesenchymal stem cells on regeneration of sciatic nerve defects in Sprague dawley rats
2024

Effects of Umbilical Cord Stem Cell Secretome on Nerve Regeneration

Sample size: 28 publication Evidence: high

Author Information

Author(s): Kurniawan Aryadi, Dilogo Ismail Hadisoebroto, Pawitan Jeanne Adiwinata, Widodo Wahyu, Oesman Ihsan, Martinus Ade

Primary Institution: Faculty of Medicine Universitas Indonesia, Department of Orthopaedics & Traumatology, Dr Cipto Mangunkusumo National Central Hospital, Jakarta, Indonesia

Hypothesis

This study aims to assess the secretome’s potency in regenerating peripheral nerve defects.

Conclusion

The secretome of umbilical cord MSC can be a novel therapy in replacing autografts for the management of peripheral nerve defects.

Supporting Evidence

  • The secretome showed comparable results to autografts in regenerating sciatic nerve defects.
  • Functional recovery was better in the secretome group compared to other treatments.
  • The number of myelinated axons was significantly higher in the secretome group.
  • The secretome prevented muscle atrophy in treated rats.
  • Secretome application is quicker and overcomes challenges associated with autografts.

Takeaway

Scientists tested a special fluid from umbilical cord stem cells to see if it helps heal nerve injuries in rats, and it worked as well as traditional methods.

Methodology

The study involved 28 Sprague-Dawley rats with sciatic nerve injuries divided into four groups receiving different treatments, including autografts and secretome.

Limitations

Not all sciatic nerve defects regenerated, and some groups showed slower recovery.

Participant Demographics

12-16-week-old male Sprague Dawley rats weighing 250–300 grams.

Statistical Information

P-Value

<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0310467

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