GATA4/FOG2 transcriptional complex regulates Lhx9 gene expression in murine heart development
2008
GATA4/FOG2 Complex Regulates Lhx9 in Heart Development
publication
Evidence: high
Author Information
Author(s): Fatima O. Smagulova, Nikolay L. Manuylov, Lyndsay L. Leach, Sergei G. Tevosian
Primary Institution: Dartmouth Medical School
Hypothesis
The GATA4/FOG2 transcriptional complex regulates the expression of the Lhx9 gene during cardiac development.
Conclusion
The Lhx9 gene is the first direct target of the GATA4/FOG2 repressor complex in cardiac development.
Supporting Evidence
- The study identified Lhx9 as a direct target of the GATA4/FOG2 complex.
- Chromatin immunoprecipitation confirmed the binding of GATA4/FOG2 to Lhx9 regulatory regions.
- Transient transfection studies showed that FOG2 represses Lhx9 expression in the presence of GATA4.
Takeaway
This study found that two versions of the Lhx9 gene are important for heart development, and their expression is controlled by a protein complex called GATA4/FOG2.
Methodology
The study used chromatin immunoprecipitation and real-time PCR to analyze gene expression and protein interactions.
Digital Object Identifier (DOI)
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