Imaging Apoptosis with Radiolabelled Annexin V
Author Information
Author(s): Collingridge D R, Glaser M, Osman S, Barthel H, Hutchinson O C, Luthra S K, Brady F, Bouchier-Hayes L, Martin S J, Workman P, Price P, Aboagye E O
Primary Institution: Cancer Research UK PET Oncology Group, Imperial College of Science Technology and Medicine
Hypothesis
Can iodinated-annexin V be used to detect apoptosis in tumors?
Conclusion
Iodinated annexin V can be used to detect chemotherapy-induced apoptosis in vitro and in tumor-bearing mice.
Supporting Evidence
- The study demonstrated that radiolabelled annexin V binds specifically to apoptotic cells.
- In vivo studies showed increased uptake of annexin V in tumors treated with chemotherapy.
- Statistical analysis indicated significant differences in binding between treated and control cells.
Takeaway
This study shows that a special protein can help doctors see if cancer cells are dying after treatment, which is important for understanding how well the treatment is working.
Methodology
The study involved radiolabelling annexin V and testing its binding to apoptotic cells in vitro and in vivo using PET imaging.
Potential Biases
Potential for binding to necrotic cells, which could confound results.
Limitations
The study did not detect tumors by PET imaging, and high uptake in kidneys may affect quantification.
Participant Demographics
C3H/Hej mice bearing RIF-1 tumors.
Statistical Information
P-Value
0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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