Detecting Changes in Phosphocholine Related to Ras Activation
Author Information
Author(s): Ronen S M, Jackson L E, Beloueche M, Leach M O
Primary Institution: Cancer Research Campaign (CRC) Clinical Magnetic Resonance Research Group, Institute of Cancer Research, Royal Marsden Hospital
Hypothesis
Can magnetic resonance spectroscopy detect changes in phosphocholine associated with Ras activation and inhibition?
Conclusion
Magnetic resonance spectroscopy can detect increases in phosphocholine levels associated with Ras activation and decreases in response to treatment.
Supporting Evidence
- Phosphocholine levels increased significantly in ras transfected cells compared to NIH 3T3 cells.
- The PC/NTP ratio also increased significantly in the transfected cells.
- Treatment with agents inhibiting Ras signalling led to a drop in phosphocholine levels in ras transfected cells.
Takeaway
This study shows that a special imaging technique can help see changes in a substance related to cancer when certain treatments are used.
Methodology
The study used 31P magnetic resonance spectroscopy to compare NIH 3T3 cells with mutant ras transfected cells.
Statistical Information
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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