Development of a rapid dipstick with latex immunochromatographic assay (DLIA) for diagnosis of schistosomiasis japonica
2011

Rapid Dipstick Test for Schistosomiasis Japonica

Sample size: 333 publication 10 minutes Evidence: high

Author Information

Author(s): Yu Li-Ling, Ding Jian-Zu, Wen Li-Yong, Lou Di, Yan Xiao-Lan, Lin Li-Jun, Lu Shao-Hong, Lin Dan-Dan, Zhou Xiao-Nong

Primary Institution: Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, China

Hypothesis

Can a new rapid dipstick test effectively diagnose schistosomiasis japonica?

Conclusion

The DLIA is a simple, rapid, and effective assay for diagnosing schistosomiasis japonica, suitable for large-scale applications.

Supporting Evidence

  • The sensitivity of DLIA was 95.10% and specificity was 94.91%.
  • DLIA showed no significant difference in detection rates compared to ELISA.
  • The kappa value between DLIA and ELISA was 0.90, indicating high agreement.
  • Field tests involved 333 serum samples from an endemic area.

Takeaway

Researchers created a quick test to find out if people have schistosomiasis, which is a disease caused by a parasite. This test is easy to use and can help many people at once.

Methodology

The study developed a dipstick test using latex microspheres to detect antibodies in human serum, comparing results with ELISA and Kato-Katz methods.

Limitations

The study did not address the cross-reactivity with paragonimiasis and the potential for false positives.

Participant Demographics

Participants were aged 6-65 years from a schistosomiasis endemic area in Jiangxi Province, China.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1756-3305-4-157

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