Repression of Mitochondrial Translation, Respiration and a Metabolic Cycle-Regulated Gene, SLF1, by the Yeast Pumilio-Family Protein Puf3p
2011
Puf3p and Slf1p Regulate Mitochondrial Respiration
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Chatenay-Lapointe Marc, Shadel Gerald S.
Primary Institution: Yale University School of Medicine
Hypothesis
How does Puf3p control mitochondrial gene expression and respiration?
Conclusion
Puf3p represses mitochondrial translation and respiration without affecting overall mitochondrial biogenesis.
Supporting Evidence
- Puf3p has a general repressive effect on mitochondrial OXPHOS abundance, translation, and respiration.
- Lack of Puf3p increased mitochondrial oxygen consumption during logarithmic growth.
- Over-expression of Slf1p resulted in a modest increase in mitochondrial oxygen consumption.
Takeaway
Puf3p is like a traffic cop that slows down how much energy-making stuff is made in yeast cells, while Slf1p helps speed it up when needed.
Methodology
The study involved analyzing mitochondrial parameters in wild-type and puf3Δ yeast strains grown in synthetic dextrose medium.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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