Cannabinoid Receptors in Murine Embryonic Stem Cells
Author Information
Author(s): Jiang Shuxian, Fu Yigong, Williams John, Wood JodiAnne, Pandarinathan Lakshmipathi, Avraham Shiri, Makriyannis Alexandros, Avraham Shalom, Avraham Hava Karsenty
Primary Institution: Beth Israel Deaconess Medical Center, Harvard Medical School
Hypothesis
CB1 and CB2 play regulatory roles in the hematopoietic differentiation of ES cells and that endocannabinoids are important for the survival of ES cells.
Conclusion
The study reveals that cannabinoid receptors CB1 and CB2 are crucial for the survival and differentiation of murine embryonic stem cells.
Supporting Evidence
- CB1 and CB2 receptors were significantly induced during the hematopoietic differentiation of murine ES-derived embryoid bodies.
- Endocannabinoids were found to be involved in the survival of murine ES cells.
- Treatment with cannabinoid ligands increased hematopoietic differentiation of ES cells.
Takeaway
This study shows that certain receptors in stem cells help them grow and change into different types of cells, which is important for making blood cells.
Methodology
The study used RT-PCR and Western blot analyses to examine the expression of cannabinoid receptors and endocannabinoids in murine embryonic stem cells and embryoid bodies.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website