IL-7 and IL-8 inhibit gamete interaction in the zona-free hamster egg sperm penetration assay
1992
IL-7 and IL-8 inhibit gamete interaction in the sperm penetration assay
publication
Evidence: moderate
Author Information
Author(s): H. Lambert, I. Collazo, A. Steinleitner
Primary Institution: Mount Sinai Medical Center
Hypothesis
The study aims to assess the effect of interleukin-7 (IL-7) and interleukin-8 (IL-8) on gamete interaction.
Conclusion
IL-7 and IL-8 significantly inhibit gamete interaction, suggesting their role in subfertility associated with endometriosis.
Supporting Evidence
- Preincubation with IL-7 and IL-8 produced a dose-dependent inhibition of fertilization.
- Addition of either cytokine at concentrations of 5 and 50 ng/ml completely inhibited gamete interaction.
- Spermatozoa preincubated in neat BWW penetrated 88% of hamster oocytes.
Takeaway
This study found that two proteins, IL-7 and IL-8, can stop sperm from reaching and fertilizing eggs, which might explain some fertility problems.
Methodology
Sperm was preincubated with IL-7 or IL-8, and gamete interaction was assessed using the sperm penetration assay.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
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