Endothelial Cell Growth Factors from Tumor Cells
Author Information
Author(s): H. Acker, F. Pietruschka, J. Deutscher
Primary Institution: Max-Planck-Institut fur Systemphysiologie
Hypothesis
Can the tissue culture model of multicellular spheroids be used to study the production of angiogenic factors by tumor cells?
Conclusion
The study found that HT29 cells in spheroid culture released significantly more endothelial cell mitogenic activity than those in monolayer culture.
Supporting Evidence
- HT29 cells in spheroid culture released a significantly higher endothelial cell mitogenic activity than in monolayer culture.
- Monolayer conditioned medium has a significantly higher mitogenic activity than control.
- The degree of endothelial cell mitogen release can be influenced by culturing cells in monolayer or spheroid culture.
Takeaway
This study shows that tumor cells grown in a special way can release more substances that help blood vessels grow.
Methodology
Human colon carcinoma cells (HT29) were grown in monolayer and spheroid cultures, and the mitogenic activity of the conditioned media was measured using 3H-thymidine incorporation in endothelial cells.
Limitations
The study does not provide detailed characterization of the released mitogen or its relation to known angiogenic factors.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
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