Barcoding Notch signaling in the developing brain
Author Information
Author(s): Siniscalco Abigail M., Perera Roshan Priyarangana, Greenslade Jessie E., Veeravenkatasubramanian Hemagowri, Masters Aiden, Doll Hannah M., Raj Bushra
Primary Institution: University of Pennsylvania Perelman School of Medicine
Hypothesis
Can CRISPR-Cas molecular recorders be used to capture Notch signaling during zebrafish brain development?
Conclusion
SABER-seq effectively records Notch signaling activity in zebrafish, allowing for the identification of cell types derived from Notch-active progenitors.
Supporting Evidence
- SABER-seq allows for the permanent recording of transient signaling events.
- Notch signaling is crucial for maintaining neural progenitor populations.
- SABER-seq enhances cell type resolution compared to traditional signaling reporters.
- Barcode editing was highest in central nervous system tissues.
- Notch activity was detected in various brain cell types derived from progenitors.
Takeaway
The study created a new tool to track how signals affect brain cell development in zebrafish, helping scientists understand brain growth better.
Methodology
The study used a CRISPR-Cas molecular recorder called SABER-seq to capture Notch signaling in zebrafish brains and analyzed the results through single-cell transcriptomics.
Potential Biases
Potential bias in the expression and amplification of the Notch recorder transgene among different cell types.
Limitations
The study's findings may not be generalizable to all signaling pathways or organisms due to the specific focus on Notch signaling in zebrafish.
Participant Demographics
Zebrafish embryos and adults were used in the study.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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