Human pancreatic cancer cell lines do not express receptors for somatostatin
Author Information
Author(s): J. Gillespie, G.J. Poston, M. Schachter, P.J. Guillou
Primary Institution: Imperial College of Science, Technology & Medicine, St. Mary's Hospital Medical School, London, UK
Hypothesis
Do human pancreatic cancer cell lines express receptors for somatostatin?
Conclusion
The study found that human pancreatic cancer cell lines MiaPaCa-2 and Panc-1 do not have somatostatin receptors, which explains why somatostatin and its analogues do not inhibit their growth.
Supporting Evidence
- Neither SS-14 nor RC-160 inhibited the growth of MiaPaCa-2 or Panc-1 cell lines.
- RC-160 inhibited the EGF-stimulated growth of a rat pancreatic cancer cell line AR42J.
- Binding studies showed no displaceable binding on MiaPaCa-2 or Panc-1.
Takeaway
The researchers looked at two types of human pancreatic cancer cells and found that they don't have the parts needed to respond to a hormone called somatostatin, which means that this hormone can't help stop the cancer from growing.
Methodology
The study involved in vitro experiments with human pancreatic cancer cell lines MiaPaCa-2 and Panc-1, assessing their growth in response to somatostatin and its analogues, and conducting binding studies to check for receptor presence.
Limitations
The study only examined three cell lines, which may not represent all pancreatic cancers.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website