Carbonic anhydrase-9 expression levels and prognosis in human breast cancer: association with treatment outcome
2003

CA-9 Levels and Breast Cancer Treatment Outcomes

Sample size: 253 publication Evidence: moderate

Author Information

Author(s): Span P N, Bussink J, Manders P, Beex L V A M, Sweep C G J

Primary Institution: University Medical Centre Nijmegen

Hypothesis

The study aims to assess CA9 expression levels in breast cancer samples and correlate them with treatment outcomes.

Conclusion

CA9 expression is associated with poor treatment outcomes in breast cancer patients, particularly in high-grade, steroid receptor-negative tumors.

Supporting Evidence

  • CA9 expression levels were higher in high-grade tumors compared to lower grades.
  • CA9 levels were significantly associated with steroid hormone receptor status.
  • Younger age and higher number of involved lymph nodes were linked to poorer relapse-free survival.

Takeaway

This study found that a protein called CA9 can help doctors understand which breast cancer patients might not respond well to certain treatments.

Methodology

The study used real-time quantitative RT-PCR to measure CA9 expression in breast cancer tissue samples and correlated these levels with relapse-free survival.

Potential Biases

Potential selection bias due to the retrospective nature of the study and treatment decisions made prior to the study.

Limitations

The study is retrospective and may be influenced by selection bias due to treatment decisions based on consensus recommendations.

Participant Demographics

Median age of participants was 60 years, with a range from 31 to 88 years; all had unilateral, operable breast cancer.

Statistical Information

P-Value

p<0.001 for steroid receptor status

Confidence Interval

95% CI for CA9 interaction with chemotherapy: 1.07–1.61; for endocrine therapy: 1.20–1.66

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1038/sj.bjc.6601122

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