How Penetratin Interacts with Cell Membranes
Author Information
Author(s): Isabel D. Alves, Cherine Bechara, Astrid Walrant, Yefim Zaltsman, Chen-Yu Jiao, Sandrine Sagan
Primary Institution: UPMC Univ Paris 06, UMR 7203, LBM, Paris, France
Hypothesis
Does the interaction of penetratin with cell surface components affect its internalization efficacy?
Conclusion
The study concludes that high-affinity binding of penetratin to cell membranes does not predict its internalization efficiency.
Supporting Evidence
- Penetratin internalized with the same efficacy in three cell lines at 1 µM.
- At 10 µM, penetratin was better internalized in SAneg cells compared to WT and GAGneg cells.
- The affinity of penetratin for WT cells was 10 nM, while it was in the micromolar range for SAneg and GAGneg cells.
Takeaway
Penetratin is a special peptide that can enter cells, but just because it sticks to the outside of the cell doesn't mean it will get inside easily.
Methodology
The study used mass spectrometry to quantify internalized and membrane-bound penetratin in different cell lines and investigated binding affinity and kinetics using plasmon resonance and calorimetry.
Limitations
The sensitivity of the methods used limited the ability to measure very low concentrations of penetratin accurately.
Participant Demographics
The study involved three different cell lines: wild type (WT), glycosaminoglycans-deficient (GAGneg), and sialic acid-deficient (SAneg) CHO cells.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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