Gut-Lymph-Induced Lung Injury in Mice
Author Information
Author(s): Reino Diego C. Pisarenko, Vadim Palange, David Doucet, Danielle Bonitz, Robert P. Lu, Qi Colorado, Iriana Sheth, Sharvil U. Chandler, Benjamin Kannan, Kolenkode B. Ramanathan, Madhuri Xu, Da Zhong Deitch, Edwin A. Feinman
Primary Institution: Department of Surgery, University of Medicine and Dentistry of New Jersey (UMDNJ)- New Jersey Medical School, Newark, New Jersey, United States of America
Hypothesis
Factors in the intestinal mesenteric lymph after trauma hemorrhagic shock mediate gut-induced lung injury via TLR4 activation.
Conclusion
Non-microbial factors in the intestinal mesenteric lymph after trauma hemorrhagic shock can recreate lung injury through TLR4 activation.
Supporting Evidence
- The infusion of porcine lymph into naïve mice induced lung injury.
- TLR4 activation was necessary for the development of lung injury.
- TLR2 activation was not involved in the pathology of lung injury.
- Endotoxin and bacterial DNA were absent in the lymph samples.
Takeaway
When mice received lymph from pigs that had experienced trauma, their lungs got hurt because of a specific pathway in their body that reacts to injury.
Methodology
Mice were infused with porcine lymph collected from pigs subjected to trauma hemorrhagic shock, and lung injury was assessed using Evans blue dye permeability and myeloperoxidase levels.
Potential Biases
Potential bias in the selection of animal models and the interpretation of results.
Limitations
The study was conducted in mice, which may not fully replicate human responses to trauma.
Participant Demographics
Adult male Yorkshire minipigs and male CD-1 mice were used in the study.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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