Permeation through the Cell Membrane of a Boron-Based β-Lactamase Inhibitor
2011

How a Boron-Based Drug Passes Through Bacterial Membranes

publication Evidence: moderate

Author Information

Author(s): Minozzi Manuela, Lattanzi Gianluca, Benz Roland, Costi Maria P., Venturelli Alberto, Carloni Paolo

Primary Institution: University “Roma Tre”

Hypothesis

The study investigates the permeation mechanism of boronic compounds through bacterial membranes.

Conclusion

The research shows that the neutral form of the boron-based β-lactamase inhibitor BZB can efficiently permeate bacterial membranes, while the negatively charged form cannot.

Supporting Evidence

  • BZB passes through the membrane in both charged and neutral forms.
  • The neutral form of BZB is responsible for the biological activity.
  • A water-filled channel assists the translocation of BZB through the membrane.

Takeaway

This study looks at how a special drug can get through the walls of bacteria. It finds that the drug works better when it's not charged.

Methodology

The study used electrophysiological experiments and molecular dynamics simulations to analyze the permeation of BZB through model membranes.

Limitations

The study primarily focuses on model membranes and may not fully represent in vivo conditions.

Digital Object Identifier (DOI)

10.1371/journal.pone.0023187

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