SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): identification of early mediators of insect responses
2010

Identifying Plant Responses to Insect Elicitation

publication Evidence: moderate

Author Information

Author(s): Gilardoni Paola A, Schuck Stefan, Jüngling Ruth, Rotter Björn, Baldwin Ian T, Bonaventure Gustavo

Primary Institution: Max Planck Institute for Chemical Ecology

Hypothesis

How fatty acid-amino acid conjugates (FACs) are perceived and activate signal transduction mechanisms in Nicotiana attenuata is unknown.

Conclusion

The study identified regulatory genes involved in insect-specific mediated responses in Nicotiana attenuata after FAC elicitation.

Supporting Evidence

  • The analysis targeted mRNAs encoding regulatory components with rapid FAC-elicited kinetics.
  • Six FAC-regulated genes were functionally characterized by Virus Induced Gene Silencing (VIGS).
  • More than 25% of the annotated UniTags corresponded to putative regulatory components.

Takeaway

When certain caterpillars attack a plant, the plant can sense it and quickly change how it behaves to defend itself. This study found some important genes that help the plant do this.

Methodology

SuperSAGE combined with 454 sequencing was used to quantify early transcriptional changes in response to FAC elicitation.

Limitations

The study primarily focused on genes with significant changes in expression, which may overlook other important factors.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2229-10-66

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