Detecting p53 Mutations in Bladder Cancer Samples
Author Information
Author(s): Phillips H A, Howard G C W, Miller W R
Primary Institution: Department of Clinical Oncology, University of Edinburgh, Western General Hospital, Edinburgh, UK
Hypothesis
Can p53 mutations be detected in bladder washing samples from patients with bladder cancer?
Conclusion
The study found that p53 mutations can be detected in bladder-washing samples from patients with bladder cancer.
Supporting Evidence
- An abnormal SSCP band was detected in five samples from five different patients.
- The same abnormal SSCP pattern was demonstrated in samples of the corresponding bladder tumour.
- In one case, bladder washings were available from the same patient on two separate occasions.
Takeaway
Doctors can find cancer genes in urine samples from bladder cancer patients, which might help them avoid more invasive tests.
Methodology
The study used single-stranded conformational polymorphism (SSCP) analysis to detect mutations in the p53 gene in bladder-washing specimens.
Limitations
The study is a pilot and may not represent larger populations.
Participant Demographics
Patients with bladder cancer (27 patients, 31 samples).
Digital Object Identifier (DOI)
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